MuHigh-Performance Liquid Chromatography (HPLC), kunyatsoita uye kunyatsoshanda kwakakosha kuti uwane mhedzisiro chaiyo. Chimwe chezvinhu zvakakosha mukuona kushanda kwakanaka kweHPLC system ndeyecheki vharafu. Kunyangwe ichiwanzo furatirwa, cheki vharuvhu inoita basa rakakosha mukudzora kuyerera kwechikamu chenhare, kuchengetedza kutendeseka kwehurongwa, nekudzivirira midziyo inonzwisisika sepombi. Muchinyorwa chino, tichaongorora kukosha kwemavharuvhu muHPLC masisitimu, mhando dzawo, mabasa, uye kukosha kwekuchengetedza kwakaringana.
Iyo Yakakosha Basa reKutarisa Mavharuvhu muHPLC
Ivhavha yekuongorora muHPLC inodzivirira kudzoreredza kusingadiwi kwezvinonyungudutsa kana nhanho dzenhare muhurongwa, kuve nechokwadi chekuyerera kunoenderana uye kwakananga. Ichi chakareruka asi chakakosha chikamu chakakosha pakuona kwakaringana, kudzokorora mhedzisiro yechromatographic. Heano kutarisisa kweakakosha mabasa evhavha yekutarisa:
1. Kudzivirira Backflow
Basa rekutanga revhavhavha yecheki ndeyekudzivirira kuyerera kwe mobile phase kana solvent. MuHPLC masisitimu, kuchengetedza inogara kuyerera kwakakosha kudzivirira kusvibiswa kana mhedzisiro. Pasina cheki valve, panogona kuva nengozi yekudzoka shure, iyo inogona kukonzera kusanganiswa kwezvinonyungudutsa, kusvibiswa kwesampuli, kana kuparadzaniswa kusina kukodzera kwemakemikari.
2. Kudzivirira Pombi
Iyo HPLC pombi chikamu chakakosha cheiyo system inoita kuti nharembozha ifambe nemukoramu pakumanikidzwa kunodiwa. Nekudaro, kana pombi yamira, kumanikidza kunogona kudonha, zvichikonzera kudzoka shure. A cheki valve inovimbisa kuti kudzvinyirira kunochengetwa kunyange kana pombi isiri kushanda nesimba, kudzivirira kukanganisa kwepombi kana kurasikirwa kwekumanikidza.
3. Kuchengetedza Kutendeseka Kwehurongwa
HPLC masisitimu anotsamira pane yakatetepa chiyero pakati pekumanikidza, kuyerera mwero, uye solvent kuumbwa. Kana iyo nzira yekuyerera ikakanganiswa nekuda kwekudzokera kumashure, inogona kukanganisa iyo yese system. Ivhavha yekuongorora inochengetedza kutendeseka kwehurongwa nekuita kuti nharembozha inoyerera chete nenzira yaidiwa, inovandudza kururama uye kuwirirana kwekuongorora.
Mhando dzeCheki Mavharuvhu Anoshandiswa muHPLC
Mhando dzakasiyana dzecheki mavharuvhu anoshandiswa muHPLC masisitimu, imwe neimwe yakagadzirirwa kuenderana nezvinodiwa zvekushanda. Heano mamwe emhando dzakajairika:
1. Spring-yakatakura Tarisa Valve
Iyo spring-loaded cheki valve ndiyo inonyanya kushandiswa muHPLC systems. Inoshandisa chitubu chekuvhara valve kana pasina kuyerera kana kana nzira yekuyerera ichidzorerwa. Rudzi urwu rwevhavha yecheki yakavimbika uye iri nyore kuchengetedza.
2. Bhora Tarisa Valve
Mukugadzira uku, bhora rinosundirwa pachigaro kudzivirira kubuda shure. Kana kuyerera kwamira, bhora rinovhara vharafu, richivharira chero reverse kuyerera. Bhora cheki mavharuvhu ari nyore uye anoshanda, achivaita sarudzo yakakurumbira kune madiki-scale HPLC masisitimu.
3. Diaphragm Check Valve
A diaphragm cheki vharuvhu inoshandisa diaphragm inochinjika kuvhara vharafu kana pasina kuyerera kuri kuitika. Iri rudzi rwevharuvhu rakanakira masisitimu anoda yakaderera-kudzvanywa, leak-proof chisimbiso, sezvo diaphragm inogona kuchinjika kuti igadzirise shanduko diki mukumanikidza.
Ndekupi Tarisa Mavharuvhu Anowanikwa muHPLC Systems?
Cheki mavharuvhu anowanzo kuiswa munzvimbo dzakasimba mukati meiyo HPLC sisitimu yekudzivirira kudzoka kumashure panzvimbo dzakakosha. Nzvimbo idzi dzinogona kusanganisira:
•Mumusoro wepombi:Chengetedza mavharuvhu anowanzo kuwanikwa mumusangano wepombi kudzivirira reverse kuyerera kweiyo solvent uye kuchengetedza kusagadzikana kudzvinyirira mukati mehurongwa.
•Mune jekiseni:Mune mamwe masisitimu, cheki mavharuvhu anowanikwa mune jekiseni kudzivirira kudzoka shure panguva yejekiseni remuenzaniso, kuve nechokwadi chekuti sampuli inounzwa nenzira kwayo muhurongwa.
Kukosha kweCheck Valve Maintenance
Kufanana nezvose zviri muHPLC system, cheki mavharuvhu anoda kugara achigadziriswa kuti ave nechokwadi chekuti anoshanda nemazvo. Nekufamba kwenguva, mavharuvhu echeki anogona kuvharwa nematehwe, kuderedzwa nezvinonyungudutsa, kana ruzivo rwekupfeka uye kubvaruka nekuda kwekushandisa kakawanda. Izvi zvinogona kutungamira kune nyaya dzakadai sekudonha, kurasikirwa kwekumanikidza, kana kuyerera kusingaenderane. Kugara uchiongorora, kuchenesa, uye kutsiva cheki mavharuvhu kunogona kudzivirira nyaya idzi, kuve nechokwadi chehupenyu hurefu hweHPLC system yako uye kuchengetedza mhando yemhedzisiro yako.
Muchidimbu, cheki valve mune HPLC system inoita basa rakakosha mukuchengetedza kuyerera kwakaringana kwechikamu chenhare, kudzivirira kudzoka shure, uye kuchengetedza zvakakosha sepombi. Nekunzwisisa basa rayo uye nekuchengetedza ichi chiri nyore asi chakakosha chikamu, unogona kuvandudza iko kurongeka, kushanda nesimba, uye hupenyu hurefu hweHPLC system yako. Kunyangwe iwe uri kuita ongororo yenguva dzose kana kushanda pane zvakanyanya kuomarara chromatographic mabasa, usatarise kukosha kweiyo inoshanda nemazvo cheki vharafu mukuona kwakaringana kuita sisitimu.
Kugara uchigadzirisa uye kunzwisisa kwemhando dzecheki mavharuvhu aripo anogona kubatsira kudzikisira nyaya uye kugadzirisa kuvimbika kweHPLC system yako.
Nguva yekutumira: Nov-07-2024