Mukuongororwa kweongororo,Yakakwira-perform yemvura chromatography (HPLC)Iyi nzira yakakosha yekuparadzanisa, inozivisa, uye inofungidzira makomendi. Nekudaro, kuwana zvinowirirana uye zvakavimbika mhedzisiro inoda zvinopfuura kungova midziyo yakakodzera - inodaOptimization. Chinyorwa ichi chinoongorora kuti ungamukurudzira sei wakoHplc kuongororaKuti uwedzere kushanda zvakanaka, kuderedza nguva yekugara, uye kunatsiridza chokwadi.
Zvinetso zvakajairika muHPLC ongororo uye maitiro ekugadzirisa
Nepo Hplc ishandiso ine simba yekuongorora, haina kana matambudziko. Nyaya dzakadaiKugadziriswa kwakashata, ruzha ruzha, uye mhedzisiro isingaenderaneinogona kukanganisa mari yekubudirira. Heano maitiro ekugadzirisa matambudziko aya akajairika:
1. Kugadzirisa Kwakashata
Imwe yezvinetso zvinowanzoitika muHPLC haina kurongeka kupatsanurwa pakati pemapepa, kazhinji nekuda kweIsiriyo DELUMN Sarudzo kana Suboptimal Flow State. Kuvandudza resolution:
• Sarudza achromatographic columnNezvakakodzeraStationary phase uye Sizele Saizikune yako Ongorora.
• gadzirisakuyerera kuyerera uye gradient mamirirokusimudzira peak mupendero uye kupatsanurwa.
• Shandisakudzora tembirichakudzikamisa nguva dzekuzvidzivirira uye nekuvandudza kubereka.
2. Baseline Drift kana ruzha
Baseline ruzha runogona kukanganisa pamwe nepamusoro pekutsvaga uye kukanganisa data re data. Iyi nyaya inowanzo kukonzerwa ne:
•Tembiricha inochinjika- Chengetedza nzvimbo yakagadzikana yemarabhareti uye shandisa column oven kana zvichidikanwa.
•Yakasvibiswa nharembozha-Shandisa yakakwirira-yakanyanya solity uye faira yako nhare chikamu usati washandiswa.
•Chisungo kusvibiswa- Gara uchichenesa uye chengetedza iyo detector, pombi, uye tubing kuti uderedze ruzha rwemashure.
3. Asingapindirani peak
Kubatanidzwa kusapindirana kunokanganisa kuvimbika kwehuwandu. Kugadzirisa izvi:
• Ita shuwaHPLC chikamu chakarongedzwa zvakanakausati washandisa.
• chengetedza aYakagadzikana kuyerera chiyerouye dzivirira kudzvanywa kuchinjika.
• OngororaSoftware Zvirongwa zvePeak Kubatanidzwa, kuve nechokwadi chinoitika uye chinofanira kubereka.
Kusarudza iyo chaiyo HPLC column
Kusarudza iyo chaiyo HPLC column iriyakakosha yekuzadzisa kupatsanurwa kwakakwana. Funga nezvezvinhu izvi paunosarudza column:
•Column kureba: Kwakareba makoramu anopa kupatsanurwa zviri nani asi kuwedzera nguva yekuongorora. Sarudza kureba uko kuyera kugadziriswa uye kumhanya.
•Column Diameter: Narrower makoramu anopa yakakwira resolution asi inoda kuwedzera kudzvinyirira. Ive nechokwadi chekuenderana neyako HPLC system.
•Chiverengero cheshuwa.
Optimizizing file mbozhanhare uye kuyerera mitengo
Iyo nhare chikamu kiyi yekubudirira yeHPLC kuongorora. Heano maitiro ekuita kuti azvisarudzire:
•Gadzirisa solvent midziyo: Zvakanaka-tune thesolvent ratiokuvandudza kupatsanurana. ShandisaGradient Eleutionyemasampu akaomarara.
•Dzora ph mazinga: Ita shuwanhare phase phinoenderana nemhando mbiri uye chikamu.
•Gadzirisa kuyerera: Yakakwirira inoyerera marero inoderedza kuongororwa nguva asi inogona kukanganisa resolution. Balance kumhanya uye kushanda zvakanaka zvichienderana nenzira yako.
Kuchengetedza uye kudzivirira kutarisirwa
Kugadziriswa kwakakodzera kunozivisakuenderana kwekuita uye kunowedzera chiridzwa hupenyu. Tevedza izvi zvakanakisa maitiro:
•Maitiro ekuchenesa: Gara uchenesejekiseni, column, uye detectorkudzivirira kusvibiswa.
•Tsiva zvinokanganisa: ShandukoZvisimbiso, mafirita, uye tubingsezvatinoda kudzivirira kubhururuka nekumanikidza kuchinjika.
•Calibrate iyo system.
Mhedziso
Optimizing HPLC Ongororo yakakosha kuti ichivandudza kushanda kwerabhoritari uye ichireva mibairo yepamusoro. Nekutaura nyaya dzakajairika sengekusarongeka kugadzirisa, ruzha ruzha, uye peak kuunganidzwa kusapindirana, uye nekusarudza kodzerombiru uye nhare mbozha, unogona kusimudzira zvakanyanya kuita kwako ongororo. Nguva dzosekuchengetedza uye nzira yekungwarira yekufungidziraichachengeta yako HPLC system ichimhanya kuPeak kushanda, kuderedza nguva yekudzvanywa uye kuona mhedzisiro chaiyo, mubereki.
Yehunyanzvi nyanzviHPLC Optimization, kusanganaChromasir-Tinotaurirana mukupaYakagadzirirwa Chromatography SolutionsKuti ubatsire murabhoritari wako anowana zviyero zvepamusoro zvekuita.
Kutumira Nguva: Mar-27-2025