Ghost Peaks muChromatography: Zvikonzero uye Mhinduro neGhost-Sniper Columns

Chromatography inzira yakakosha mukutsvagisa kwesainzi kwazvino, asi kubuda kweghost peaksmune chromatograms inogona kuunza matambudziko makuru kune vanoongorora. Aya mazinga asingatarisirwe, anowanzo simuka panguva yekuparadzaniswa kwechromatographic, kunyanya mune gradient modhi, kukanganisa kuongororwa kwehuwandu uye kutambisa nguva yakakosha. Kunzwisisa zvinokonzeresa ghost peaks uye kusimudzira mhinduro nyowani seChromasir'sGhost-Sniper Columninogona kushandura maitiro ako ekuongorora.

Chii Chinonzi Ghost Peaks uye Nei Dzichikosha?

Ghost peaks zviratidzo zvisingazivikanwi muchromatogram izvo zvinokanganisa kujeka kwemigumisiro yekuongorora. Kunyange zvazvo zvingaita sezvidiki, mhedzisiro yavo inogona kuve yakakosha:

1. Quantitative Matambudziko

Kana ghost inokwira ichipindirana nepamusoro pechido, ivo vanoomesa iyo chaiyo quantification yeanalytes. Izvi zvinogona kutungamirira mukukanganisa kududzira kwedata uye mhedzisiro isina kuvimbika.

2. Nguva Inopedza Troubleshooting

Kuziva kwakabva zvipoko zvepamusoro zvinowanzoda kuferefetwa kwenguva refu, zvichitsausa pfungwa dzevaongorori kubva kumabasa akakosha. Nguva inopedzwa mukugadzirisa nyaya idzi inogona kuwedzera budiriro uye tsvakiridzo.

Ko Ghost Peaks Inobva Kupi?

Kuti unyatsobvisa ghost peaks, zvakakosha kuti unzwisise kwaakabva. Aya mazinga asingatarisirwe anowanzo simuka kubva kune zvinosvibisa mu:

1.System Zvikamu:Zvisaririra muchromatographic system zvinogona kubatsira kune ghost peaks.

2.Columns:Kusachena mumidziyo yekurongedza kana kupfeka kubva mukushandiswa kunogona kukonzera kusvibiswa.

3.Mienzaniso:Sampuli dzakasvibiswa dzinounza makomisheni asingatarisirwe muchromatogram.

4.Nharembozha:Kusachena kubva kune zvinonyungudutsa, munyu webuffer, kana aqueous/organic phases zvinowanzokonzera nhongonya dzezvipoko.

5.Containers:Sample mabhodhoro ekugadzirira uye zvimwe midziyo zvinogona kuunza zvakasara zvinosvibisa.

Mhinduro yeChimurenga: Iyo Ghost-Sniper Column

Chromasir'sGhost-Sniper Columnimhinduro yekuchinja-mutambo yakagadzirirwa kurwisa ghost peaks. Iyi chizvarwa chechipiri chinyorwa chinoratidzira chimiro chakagadziridzwa uye zvepamberi zvekurongedza, zvichiita kuti ikwanise kubata ghost peaks kunyangwe pasi pemamiriro akanyanya. Kubudirira kwayo kunoita kuti ive chishandiso chakakosha chekusimbisa nzira uye kuongorora kuongorora.

Marabhoritari anoshandisa iyo Ghost-Sniper Column ataura kuvandudzwa kwakakosha mumhando yechromatogram, kuderedzwa nguva dzekugadzirisa matambudziko, uye kuwedzera kubereka kwese.

Maitiro eKukwirisa Mabhenefiti eGhost-Sniper Columns

Kuti uite zvakakwana, tevera matanho ekudzivirira uye maitiro akanaka:

1.Kugadzirisa Nguva Yakakwana:

Wedzera 5-10 maminetsi enguva yechiyero muHPLC masisitimu kuti agamuchire vhoriyamu yekoramu.

2.Kutanga Setup:

Geza makoramu matsva ne100% acetonitrile pamwero wekuyerera we0.5 mL/min kwemaawa mana usati washandiswa kuti uve nechokwadi chekushanda kwakachena uye kwakanaka.

3.Bata Ion-Pair Reagents nekuchenjerera:

Ion-pair reagents muchikamu chenhare inogona kushandura nguva dzekuchengeta uye maumbirwo epamusoro. Shandisa nekuchenjerera kana ma reagents akadaro aripo.

4.Tsiva Makoramu Nguva Dzose:

Column hupenyu hunoenderana nezvinhu zvakaita senge mobile phase kuchena, solvent mamiriro, uye kuchena kwemidziyo. Kuchinja nguva dzose kunovimbisa migumisiro inowirirana.

5.Flush Salt-Containing Mobile Phases:

Shandisa 10% organic phase solution (semuenzaniso, methanol kana acetonitrile) usati uye mushure mekuita nharembozha dzine munyu kudzivirira kuvharika.

6.Chengeta Zvakakodzera Panguva Yekuzorora:

Kuti uchengetedze kwenguva refu, chengeta mbiru mu70% organic aqueous solution (methanol kana acetonitrile). Flush ne100% acetonitrile usati washandisazve kudzoreredza kuita.

7.Monitor Performance:

Tsiva iyo column kana iyo yekutora maitiro yakadzikira kana kana analytical zvinoda kupfuura kugona kwayo.

Nei Ghost-Sniper Columns Yakakosha kune Yako Lab

Iyo Ghost-Sniper Column inopfuura chishandiso chekugadzirisa matambudziko; inowedzera kushanda kwese kwechromatographic system yako.

•Inobvisa Ghost Peaks:Kunyangwe mumamiriro ezvinhu akaoma zvikuru, iyi koramu inobata ghost peaks zvinobudirira.

•Inodzivirira Midziyo:Inosefa kunze kwakasimba zvimedu uye organic zvinosvibisa, kuchengetedza chromatographic zviridzwa nemakoramu.

•Inovandudza Hunhu hweData:Nekubvisa kupindira, iyo column inoburitsa yakachena, yakavimbika machromatogram.

Maxi Scientific Instruments: Mumwe Wako muAnalytical Excellence

At Maxi Scientific Instruments (Suzhou) Co., Ltd., tinopa mhinduro itsva dzekugonesa marabhoritari pasi rese. Yedu Ghost-Sniper Columns dzakagadzirwa zvine hungwaru kugadzirisa matambudziko ezvipoko zvepamusoro, kuve nechokwadi chemhedzisiro uye yakatsetseka mafambiro ebasa.

Simudzira Yako Chromatography Nhasi

Usarega zvipoko zvichikanganisa tsvagiridzo yako. Chengetedza mari muChromasir's Ghost-Sniper Columns uye unzwe mutsauko mukurongeka, kushanda nesimba, uye rugare rwepfungwa. ContactMaxi Scientific Instruments (Suzhou) Co., Ltd.nhasi kuti udzidze zvakawanda nezvekuti mhinduro dzedu dzinogona sei kukwidziridza mashandiro erabhoritari yako. Pamwe chete, ngatiwane kugona mune yega chromatogram.