Ghost-Sniper Column Chromasir HPLC UPLC column inobvisa ghost peaks
Ghost-sniper column inogadzirwa kuti ibvise ghost peaks. Ghost peaks ndeyekusingazivikanwe kwakabva muchromatogram, inowanzoonekwa panguva yekuparadzaniswa kwechromatographic mu gradient elution kana kushanda kwenguva refu. Kuitika kweghost peaks kune simba rakadzama pakuedza kwevanoongorora. Ghost-sniper column inoitika kugadzirisa matambudziko iwayo uye kuita kuti maitiro ekuyedza ave nyore uye anoshanda. Iyi koramu inogona kuiswa mune yakanyanyisa mamiriro uye inoratidza yakakura yekubata maitiro. Iyo ichokwadi nzira yakanaka yekubvisa kupindira kubva kune ghost peaks pane nzira yekuongorora uye kuteedzera kuongororwa kwezvinhu.
| Chikamu Nha. | Dimension | Volume | Application |
| MC5046091P | 50 × 4.6mm | anenge 800ul | HPLC |
| MC3546092P | 35 × 4.6mm | dzinenge 580ul | HPLC |
| MC5021093P | 50 × 2.1mm | anenge 170ul | UPLC |
| MC3040096P | 30 × 4.0mm | anenge 380ul | HPLC Yakaderera chikamu vhoriyamu |
Installation
Application uye Migumisiro
1. Kana iyo batch yekuongorora yakarongeka muhurongwa hweHPLC, zvinokurudzirwa kuwedzera imwe nguva yekuenzanisa kweinenge 5 min - 10 min mumamiriro ako echromatographic, nokuda kwekuita kwehuwandu hweghost-sniper column.
2. Pamakoramu matsva, apise ne 100% acetonitrile pamwero wekuyerera we0.5ml/min kwemaawa mana usati washandiswa.
3. Ion-pair reagents mu mobile phase, inogona kutorwa ne ghost-sniper column, iyo inogona kukanganisa nguva yekuchengeta uye chimiro chepamusoro chechinangwa chako. Ndapota shandisa nekuchenjerera pasi pechikamu chakadaro chenhare.
4. Iyo column hupenyu hunoenderana nemamiriro ekuongorora, senge mobile phase. iyo inonyungudutsa yakachena, uye yakasvibiswa yemidziyo. Ndokumbirawo tsiva iyo ghost-sniper column nguva nenguva kuti ive nechokwadi chekuita.
5. Kutsiva ghost-sniper column inokurudzirwa kana kubata maitiro kunowedzera kuipa kana zvinodiwa zvisingagone kugutsikana.
6. Sechikamu chekuchenesa kune liquid chromatograph, ghost-sniper column inogona kusefa zvimedu zvakasimba uye kubvisa zvinosvibisa organic pamberi pejekitori. Iyo ghost-sniper column inopawo zviridzwa uye chromatographic koramu ine dziviriro iri nani, uye inogonesa chromatogram.
7. Kana mobile phase ine buffer salt, before and after use, transmission to flush ne 10% organic phase solution (10% methanol kana acetonitrile), kudzivirira buffer salt kunze uye kuvhara column.
8. Ndokumbira utarise kuti haasi ese epamusoro ezvipoko anogona kutorwa neGhost-Sniper column.
9. Kana iyo mbiru inosiya isina kushandiswa kwenguva yakareba, inokurudzirwa kuichengeta mu organic aqueous solution (70% methanol kana acetonitrile). Uye ndapota shandisai ne 100% acetonitrile pakuyerera kwe 0.5 ml / min kweawa 1 usati washandisa.
